The 2% agarose gel of the binding experiments.
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Samples were loaded on a 2% agarose gel in TAE (1x) buffer. Lanes containing Nupr1 + non-damaged-DNA (lane 6) and MSL1 + Nupr1 + non-damaged-DNA (lane 7) were clearly delayed from DNA control lane (lane 3), indicating that there is binding between the molecular partners. No difference was observed in MSL1 + non-damaged-DNA complex (lane 5). Lanes loaded with only protein (lanes: 1 – isolated MSL1; 2 – isolated Nupr1; and 4 – MSL1 + Nupr1 complex) showed no signal.
创建时间:
2016-02-23



