Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in T. aestivum and B. distachyon
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Novel microRNAs uncovered by deep sequencing of small RNA transcriptomes in T. aestivum and B. distachyon
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2009-06-07
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we performed microRNA deep sequencing, analyzed the microRNAs and revealed the function of the corresponding target genes, as the first step to understanding the role of microRNAs in S. officinarum. 268 candidate miRNAs were screened from sequencing microRNA library with the total RNA from sugarcane leaves using the RNA-seq ESTs of all tissue of Saccharum officinarum from our group， public sugarcane ESTs from NCBI and the reference sorghum genome from Phytozome V9. After further analysis, 43 homologous miRNAs and 9 novel miRNAs were identified. Out of the 52 miRNAs, 6 were randomly chosen and verified by qRT-PCR and the putative miRNA targets’ function was analyzed. The identification of miRNAs and their targets not only will enable to understand the roles of miRNAs in sugarcane development and growth, but also enhances the understanding of miRNA-dependent mechanisms in highly polyploid species. Identification and Characterization of microRNAs from Saccharum officinarum L by deep s
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