PCR and PSQ assays designed for characterization of influenza A(H1N1)pdm09 virus.

ANucleotide numbering of HA is relative to the first codon after the signal sequence.BAa numbering of HA is relative to the H1 mature protein of A/California/07/2009.CPrimer was used for PSQ analysis of viral HA gene in 2009/2010 with a cyclic dispensation order (ACGT)10 in SQA mode (SF662).DPrimer were used for PSQ analysis of viral HA gene in 2010/2011 with a cyclic dispensation order (ACGT)10 in SQA mode (SF662a, SF663) and a customized dispensation order ATATGTAT(ACGT)5 (SF663). The PSQ primer SF663 was also described recently [39].