Single-cell RNA-seq (10X Genomics Chromium) of LGE, MGE, CGE, and Cortex from mouse embryonic forebrain

We performed single-cell RNA sequencing to characterize transcriptional heterogeneity of neural progenitors within the ventricular zone and subventricular zone of E12.5 and E14.5 mouse cortex and ganglionic eminences. By using a transgenic mouse line (Nestin-d4-Venus) to enrich for VZ cells, we detect significant transcriptional heterogeneity within VZ and SVZ progenitors, both between forebrain regions and within spatial subdomains of specific GEs. LGE, MGE, CGE, and cortex from E12.5 and E14.5 mouse embryos.