Additional file 2 of Transcriptomic analysis provides insights into molecular mechanisms of thermal physiology

Additional file 2: Table S1. Significant Acclimation effect on mRNA Expression within each Population. Population differential expression.with FDR p<0.05. Differentially expressed genes among population pairs within 12°C or 28°C with FDR p<0.05. Table S2. Population differential expression for each acclimation temperature with FDR <0.05 . Differentially expressed genes between 12°C and 28°C within populations with FDR p<0.05.Temperature differential expression. Differentially expressed genes between 12°C and 28°C within populations with FDR p<0.05. Table S3. Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways within Significant Modules. Module identifier (Module), correlated trait(s), enriched KEGG terms, and KEGG pathways are listed in columns. Enriched KEGG terms with FDR p-value < 0.05 were mapped to KEGG pathways using KEGG Mapper. Terms of interest based on relationship to correlated trait(s) are underlined. Table S4. Enriched Gene Ontology (GO) Terms within Significant Modules. Modules are named based on the tissue they are in and trait they are significantly correlated with. Enriched GO terms with FDR p-value < 0.05 are listed. Terms of interest based on relationship to correlated trait(s) are underlined. Table S5. Primers and barcodes for 3’ mRNA library preparation. First strand synthesis with i7 primers added i7 indicies. Second strand synthesis added i5 index to 96 pooled samples. Table S6. Tn5 loading and PCR amplification primer sequences. The lower-case nucleotides complement the Tn5MErev primer. The upper-case underlined nucleotides match with the i5 (R1) and P7 match with the i7 (part of the NVdT primer).