Chromatin accessibility of T-cell acute lymphoblastic leukemia (T-ALL) cells with depletion or inhbiiton of cBAF complex [ATAC-seq]

Using ATAC-seq of the cells with cBAF-targeting gRNA or inhibitor, we identified chromatin loci whose accessibility was regulated by cBAF complex For gene depletion, Cas9-expressing cells were lentivirally transduced with gRNA and selected with puromycin for 4 days. Cells were harvested on day 10 after gRNA transduction. For inhibitor treatment, cells were treated with 1 uM BRM014 for 6 hours or 8 days.