PTPσ Novel Object Recognition

<b>Novel Object Recognition test.</b> Male and female mice 3-4 months old were used for these experiments. NOR test was performed in a transparent arena (39x20x16 cm) where two identical objects (white plastic ping-pong balls glued to 50-ml Falcon tube caps) holders at the bottom) were located (adapted from (Casarotto et al., 2021)). The animals were allowed to explore the objects for 15 min in 3 consecutive days (training sessions). At the test session (4 hours or 5 days after the last training session to assess short-term or long-term memory, respectively) one of the old objects was replaced by a new one (black rubber squash ball glued to 50-ml Falcon tube caps), and the animals were allowed to explore both objects for 5 min. The number of interactions with the objects was calculated, and the difference between the visits to the new object and the old object (fB-fA) was used to assess memory. Total number of visits (fA+fB) was used to assess total exploratory activity. Two different cohorts of animals were used to assess short-term and long-term memory.