Genes that regulate the response to SHP1 inhibition in bone marrow-derived macrophages

As an unbiased approach, RNA sequencing was used to examine the transcriptome of macrophages after CD47-SIRP-alpha axis blockade. Therefore, murine bone marrow-derived macrophages were incubated with functionalized single-walled carbon nanotubes loaded with fluorescent Cy5.5 probe and the small-molecule inhibitor (NSC-87877 from Sigma-Aldrich) of SIRP-alpha's downstream effector molecule Src homology 2 domain-containing phosphatase-1 (SHP-1). This system is referred to as SHP1i, whereas the vehicle system is referred to as SWNT. After 24 hours, macrophages were sorted by flow cytometry to isolate Cy5.5-positive macrophages in each group, which were then subjected to RNA sequencing.