Genome-wide profiling of KLF10 binding loci in mouse liver

The mammalian circadian timing system and metabolism are highly interconnected, and disruption of this coupling is associated with negative health outcomes. Krüppel-like transcription factors (KLFs) govern metabolic homeostasis in various organs and many members show a circadian expression. We previously showed that the transcription factor Kru¨ppel-like factor 10 (KLF10) is a clock-controlled gene and a regulator of gluconeogenesis in liver. To further decipher the role of KLF10 in hepatic circadian physiology and metabolism we have analyzed the binding of KLF10 to its targets in the liver at the genome scale using a combination of chromatin immunoprecipitation and deep sequencing (Chip-seq). Male mice were sampled at ZT9 corresponding to maximal expression of KLF10. Our results show that KLF10 binds to a large set of genomic targets in the liver. Further, integration of these Chip-seq data with expression data indicates that KLF10 regulates genes involved in the metabolism of lipids, carbohydrates, amino acids and nucleotides.