Distinct Circulating Expression Profiles of Long Non-coding RNAs in Heart Failure Patients with Ischemic and Non- ischemic Dilated Cardiomyopathy

Ischemic cardiomyopathy(ICM) and dilated cardiomyopathy(DCM), with distinct long-term prognosis and responses to treatment, are two major problems that lead to heart failure(HF) ultimately. In this study, we investigated the lncRNA and mRNA expressions in the plasma of patients with DCM and ICM and analyzed the different lncRNA profile between the two groups. These findings revealed for the first time the specific expression pattern of both protein-coding RNAs and lncRNAs in plasma of HF patients due to DCM and ICM which may provide some important evidence to conveniently identify the etiology of myocardial dysfunctions and help to explore a better strategy for future HF prognosis evaluation. 20 peripheral fasting blood samples were donated by chronic heart failure patients with DCM (n=11) and ICM (n=9). Plasmas were analyzed by RNA microarray (Arraystar Human LncRNA microarray, v4.0, containing 40173 lncRNAs and 20730 coding transcripts). Inclusion criteria were as follow: (1)All patients had ventricular dilation and LVEF<50%. (2)Patients with ICM were diagnosed by coronary angiogram(CAG) or suffered from myocardial infarction before. (3)All patients with DCM were proved non-ischemic by CAG or coronary CTA and in the absence of hypertensive, valvular or congenital heart diseases. Patients with the following conditions were excluded from the study: (1)Cardiac resynchronisation/modulation therapy in 3 months, (2)Acute myocardial infarction in 3 months, (3)coronary artery bypass grafting in 3 months, (4)malignant tumor or immune diseases were excluded.