RNA-seq analysis of transcriptomes in control and LAMP2A-overexpressed D3 ES cells

The role of autophagy and metabolism in regulating the pluripotency of stem cells is not well understood. LAMP2A is a key gene in CMA. Here we compared transcriptomic changes in LAMP2A-overexpressed D3 versus control cells, as well as changes in LAMP2A-overexpressed cells with versus without DM-αKG treatment (4mM, 72 hrs). We found that overexpression of LAMP2A leads to decreased expression of genes related to stem cell differentiation, and this can be largely restored by DM-αKG supplement. This study provides a promising link for autophagy, metabolism, and pluripotency of stem cells. RNA-Seq was performed to measure transcriptomes of D3 ES cells under three conditions: 1) cells stably expressed pCDH-control vector (VT), 2) LAMP2A overexpressed cells without (LAMP2A), and 3) with DM-αKG treatment (4 mM, 72 hrs) (LAMP2A+ DM-αKG).