Streptomyces from rhizospheres and compost

This information was obtained in order to demonstrate the presence of actinobacteria in different rhizospheres and in a compost system, which would inhibit the growth of bacteria resistant to some antibiotic. In addition, the aim of this study was to identify by molecular methods and phylogenetic analysis the species of native bacteria with antimicrobial activity, mainly of the Streptomyces genus, recovered from these environments.

In these data it is possible to see the number of isolates obtained from different rhizospheres and from a composting system, the culture media used for their isolation and the antimicrobial activity against antibiotic resistant and non-resistant bacteria; this activity was initially measured by direct antagonism by perpendicular streak technique. In the photo of the preliminary test, some isolates with inhibitory potential can be identified according to the space without growth between the streak of the native organism and the streak of the control bacteria.

An initial enzyme profile of all the isolates obtained is also presented, which allowed the physiological characterization of native Streptomyces with antimicrobial activity. A biochemical analysis was carried out through conventional identification tests and the microscopic and macroscopic morphological characteristics are reported, the latter analyzed through a comparison of the color of the colonies obtained in different culture media.

The data also shows the percentages of inhibition obtained with the active isolates against bacteria resistant to antibiotics. These calculations were made through the perpendicular streak method comparing the area that presented growth of the control microorganism and the areas where it was inhibited.

The native isolates that showed activity were identified through molecular tests and phylogenetic analysis, for which sequences reported in GenBank for the 16S ribosomal gene were organized and shown in the respective sequence bank file.