A global genetic interaction network maps a wiring diagram of cellular function

INTRODUCTION: Genetic interactions occur when mutations in two or more genes combine to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic interaction occurs when two mutations, neither lethal individually, combine to cause cell death. Conversely, positive genetic interactions occur when two mutations produce a phenotype that is less severe than expected. Genetic interactions identify functional relationships between genes and can be harnessed for biological discovery and therapeutic target identification. They may also explain a considerable component of the undiscovered genetics associated with human diseases. Here, we describe construction and analysis of a comprehensive genetic interaction network for a eukaryotic cell. RATIONALE: Genome sequencing projects are providing an unprecedented view of genetic variation. However, our ability to interpret genetic information to predict inherited phenotypes remains limited, in large part due to the extensive bu..., , , , # Data from: A global genetic interaction network maps a wiring diagram of cellular function

Dataset DOI: [10.5061/dryad.4291s](10.5061/dryad.4291s)

## Description of the data and file structure

#### Data File S1. Raw genetic interaction datasets - Pair-wise interaction format

This folder contains complete SGA genetic interaction data for the following: • A list of all query and array mutant strains represented in the genetic interaction network along with their corresponding fitness estimates • Nonessential x Nonessential network (NxN) • Essential x Essential network (ExE) • Essential x Nonessential network (ExN) • Genetic interactions involving DAmP alleles of essential genes • Genetic interactions for HSP90 and corresponding controls The interaction datasets are provided in a tab-delimited format with 11 columns: • Query ORF • Query gene name 40 • Array ORF • Array gene name • Array Type (DMA or TSA) and Temperature (26o C or 30o C) • Genetic interaction score (ε) • P-value • Que...