Hordeum vulgare Transcriptome or Gene expression

Total RNA was extracted from immature spikes at the awn primordium stage of cultivated barley (Hordeum vulgare L.). Total RNA was measured by using Agilent 2100 Bioanalyzer (Agilent Technologies, Technologies, PaloAlto, CA, USA) and used for construction of sequencing library. Strand-specific RNA libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) following the instructions in the TruSeq Stranded mRNA Sample Preparation Guide Rev.E (Illumina). Poly(A)-RNA was fragmented and single-strand and double-strand cDNA were synthesized. High-throughput sequencing was conducted using HiSeq 2500 (Illumina, San Diego, CA, USA).