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Additional file 5 of DNA polymerases in precise and predictable CRISPR/Cas9-mediated chromosomal rearrangements

NIAID Data Ecosystem2026-05-01 收录

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https://figshare.com/articles/dataset/Additional_file_5_of_DNA_polymerases_in_precise_and_predictable_CRISPR_Cas9-mediated_chromosomal_rearrangements/26651508 数据链接链接失效反馈

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Additional file 5: Table S3. Supporting data values.

应用场景：

创建时间：

2023-12-08

CRISPR-Cas9基因编辑

sgRNA优化

The current commonly used single-guide RNA (sgRNA) structure has a shortened duplex compared with the native bacterial clustered regularly interspaced short palindromic repeats RNA (crRNA)–transactiva

NIAID Data Ecosystem110

Supplementary Material for: One-step leaping evolution from an autosomal pair to the heteromorphic sex chromosomes

染色体进化

染色体结构变异

Background: Sex chromosomes evolve from an autosomal pair after the acquisition of a sex-determining gene. The primary sex chromosomes are homomorphic in both sexes and often undergo heteromorphism in

DataCite Commons2025-05-01 更新50

Application of iGUIDE to identify potential off-target cleavage caused by Cas9/PD1 sgRNA

CRISPR-Cas9基因编辑

CAR-T细胞疗法

We successfully develop a two-in-one approach to generate non-viral genome specific targeted CAR T cells through CRISPRCas9. In the adoptive therapy for relapsedrefractory aggressive B-cell non-Hodgki

NIAID Data Ecosystem40

Additional file 1: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

CRISPR-Cas9基因编辑

小鼠遗传模型分析

Table S1. The guide RNA and singlestranded Oligonucleotide DNA sequences, their concentrations and the length of genomic regions floxed (in bp) reportedÂ in this study. (XLSX 29 kb)

Figshare2024-02-15 更新80

Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration

CRISPR-Cas9基因编辑

非病毒靶向基因组整合

We developed Cas9 fusion proteins containing single-stranded DNA-binding proteins and a 20-amino acid motif with single-stranded DNA-binding function. These fusion proteins demonstrated higher efficie

NIAID Data Ecosystem40

Additional file 5 of DNA polymerases in precise and predictable CRISPR/Cas9-mediated chromosomal rearrangements

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