Control of osteoblast regeneration by a train of Erk activity waves

We report here a dataset regarding the transcriptome of Erk active osteoblasts in regenerating zebrafish scales. We recently discovered that the marker osx:Venus-hGeminin anti-correlates with Erk activity in those cells. Thus, by sorting (using FACS) cells based on both the expression of an osteoblast marker (osx::H2A-mCherry) and the levels of hGeminin, we could sort osteoblasts into separate Erk+ and Erk- populations and perform RNAsequence (in triplicates). We found that dusp and sprouty transcripts are elevated in osteoblasts with high Erk activity. Specifically, dusp2, dusp5 and spry4 transcripts are predominant in scales and consistently present at higher levels in the population enriched for Erk+ cells. Comparison of the transcriptome of osteoblasts of regenerating zebrafish scales with high and low Erk activity. Cells are sorted on the basis of their osx:Venus-hGeminin signal, which anti-correlates with Erk activity.