Figure S1 - Levodopa-Induced Dyskinesia Is Associated with Increased Thyrotropin Releasing Hormone in the Dorsal Striatum of Hemi-Parkinsonian Rats

Pre-absorption of anti pYE17 antibody demonstrates the specificity of proTRH immunostaining. For this experiment we used a rat with a 6-OHDA lesion and long-term levodopa treatment. This animal developed LIDs and upregulation of TRH. We immunostained striatal sections from this rat with anti-pYE17 alone, anti-pYE17 pre-absorbed with either pYE17 or pYE27 peptide or with the primary antibody omitted. (A) Anti-pYE17 alone shows strong immunostaining for proTRH. (B) Immunostaining was completely blocked by preabsorbtion of anti-pYE17 with pYE17 peptide. (C) Immunostaining for proTRH was not disrupted by preabsorbtion of anti-pYE17 with pYE27 peptide. (D) Omission of the primary antibody anti-pYE17 abolished immunostaining.
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