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miRNA expression in EBCs. miRNA expression in EBCs

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA847981
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We analyzed expression of miRNAs in Exhaled Breath Condensates from pediatric patients with sickle cell disease, asthma, sickle cell disease and asthma, and controls Overall design: We extracted RNA from 0.5 mL of EBCs using the Plasma/Serum RNA Purification Kit. The expression of 1,066 human miRNAs was quantified using the Human miRNome miScript® miRNA PCR Array (QIAGEN, MIHS-3216Z). Results (Ct values) were normalized to global means, and fold changes were calculated using the 2-ΔΔCT method. SCD: 1,2,3,4,5,6,7,8,9 SCD+ASTHMA: 10,11,12,13 CONTROL: 14,15,16,17,18,19,20,21,22 ASTHMA: 23,24,25,26
创建时间:
2022-06-10
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