A multi-omic analysis of an Enterococcus faecium mutant reveals genetic mutation and dramatic changes of mRNA and protein expressions

Three iTRAQ8plex experiment replicate using TripleToF 5600 system. Protein identification were performed using the ProteinPilot software (version 4.0.8085,Paragon Algorithm version 4.0.0.0).All MS/MS spectra was search against amino acids sequences which is annotated from genome. The search parameters was set as follows: Cys. Alkylation: Iodoacetic acid, Digestion: Trypsin, ID Focus: Biological modifications, Search Effort: Thorough. The search results was filter using Local FDR 5%.