Detection of T. cruzi antigen and DNA in urine samples and kidney injury during infection.

aCorresponds to the detection of bands of 75, 80, 120 and/or 150 kDa.bParasites in kidney were detected by H&E stain or PCR. In order to be considered positive one or more parasites had to be observed in two entire tissue sections.cObservation of three or more of the following histopathological changes: inflammation, necrosis, fibrosis, vasculitis, congestion, fibrosis and mesangial hypercellularity.dIncrease in level of two or more biochemical parameters: serum urea, urine proteins and urine KIM-1.eAcute phase: n = 40 samples analyzed.fChronic phase: n = 19 samples analyzed.gResults obtained using primers TcZ1/TcZ2.