CD146-based cell sorting and telomere length in umbilical cord

UC MSC and fibroblast cultures were evaluated for their expression levels of selected transcripts characterizing multiple cell fates in vivo. Ct values obtained using Taqman qPCR technology are shown in dataset a (UC=Umbilical cord; FIB=fibroblast cell lines). The flow cytometry phenotype profiles of UC MSCs (cultures tested against a panel of MSC markers) and UC ECs (cultures tested against a panel of EC marekers) are shown in dataset b. Telomere lengths of cultured UC MSC1 and MSC2 samples are displayed in dataset c (telomere lengths calculated as T/S ratio in different passages of UC MCS cultures). Telomere lengths as T/S ratio and calculated length of freshly sorted cells are shown in dataset d (Telomere length was calculated as T/S ratio and length in bp from the freshly sorted CD45-CD235α-CD31-CD146+ (candidate MSCs) and donor-matched CD45-CD235α-CD31-CD146- (non-MSCs) of n=7 UCs).