NCBI Organism:Mus musculus, ; Cell Types:, , ;

<b>MiniSOG+ ipRGC axon labeling in the intergeniculate leaflet (IGL)</b><br>We have labeled ipRGCs with a genetically encoded and membrane-tethered version of miniSOG and systematically mapped their axonal arbors within 3D SBEM volumes of mouse ON, SCN, OPN, vLGN, IGL, and dLGN. Although ipRGCs have been classified into up to 6 different subtypes based on their dendritic arbors in the retina, there is no conclusive evidence that any subtype specifically and exclusively targets any brain region. By quantitatively determining the ultrastructural features of ipRGC synaptic contacts and intracellular machinery within these volumes, we discovered salient target-specific synaptic properties of ipRGCs.

我们对基因编码的、膜结合型 MiniSOG 进行了 ipRGC 标记，并系统地绘制了其神经轴突在 3D SBEM 大鼠视神经、下丘脑、瞳孔括约肌、外侧膝状体、中间神经叶和内侧膝状体体积内的分布。尽管根据视网膜上的树突分支，ipRGCs 已被划分为多达 6 个不同的亚型，但目前尚无确凿证据表明任何亚型能特异性且专一地靶向特定的脑区。通过定量分析这些体积内 ipRGC 突触接触和细胞内机制的亚细胞结构特征，我们发现了 ipRGCs 的显著靶向特异性突触特性。