Next Generation Sequencing analysis of the global expression profiling in bone marrow derived macrophages response to porcine circovirus type 2.

To know the influences of PCV2 infection on macrophage phenotype, we examined the expression profiling of global genes in PCV2 infected-BMDMs by RNA-seq. The results revealed that expression of 369 genes were greater than twofold in PCV2 infected-BMDMs relative to their expression levels in control cells. In addition, the expression of 201 genes were lower than in PCV2-infected macrophages compared to that of control cells . Among these genes, we found that PCV2 infection strikingly induced expression of a subset of M1-associated genes, whereas decreased expression of multiple M2-associated genes, indicating that PCV2 infection likely reprograms the macrophage transcription phenotype. mRNA profiles of BMDMs left uninfected or infected with PCV2 for 12 h.