Supplemental data for thesis

Supplemental data for thesis submitted to Cardiff University School of Biosciences:Cepraga, A.A. (2020) "An investigation into Mef2-interacting proteins during embryonic muscle development of Drosophila"Each file is provided twice: in a plain-text format such as .tsv , and as an Excel file. Note that .tsv files are tab-delimited. Make sure that they are not treated as space-delimited when imported in spreadsheet software to display them properly.This repository contains:- Table S1: Raw spectral counts and hit ranking of all proteins identified by TAP/MS (total spectral counts for each protein identified by MS in tandem affinity purification from Drosophila embryos with Mef2 as the tagged bait)- Table S2: Mef2 GO biological process terms- Table S3: High-interest candidates identified by each analysis- Table S4: Overlap of TAP datasets with muscleRNAi screen by Schnorrer et al 2010- Data supplementals for individual figures. The file names indicate which figure the file corresponds to. Most of these report the IDs of genes in the different overlap fields of Venn diagrams. The dataset for Fig4.12 contains the functional similarity network including the pairwise functional similarity scores between the candidates.- The file for Fig4.8 contains pmax for all candidates across embryonic stagings. The file for Fig4.10 contains GO enrichment for all Mef2 GO terms in each dataset and subset. These two files are in the appropriate format for use in the Morpheus matrix visualisation tool at https://software.broadinstitute.org/morpheus/ (Download the .gct files from here, open the Morpheus page, click "select file" and select the .gct file from your Downloads folder.)- a Morpheus color scheme file to be used in conjunction with Fig4-10GOenrichment[...].gct. To apply the colour scheme, first download both the Fig4-10.gct and the color-scheme.json files. Open the Fig4-10.gct in Morpheus as described above. Click the Options button in the top-right corner (cogwheel symbol), which opens the Color scheme dialog. Scroll down to find the field "Saved color scheme" and select "My computer" from the dropdown box. Finally, click "Load color scheme" and select the downloaded .json file from your Downloads folder.