The efficiencies of first double-crossover recombination and counter-selectiona.

aData are means ± SD from three independent experiments for genetic manipulations.bccpN*, point mutation of ccpN gene (G130T); ileS*, point mutation of ileS gene (G1399A); ΔccpN, in-frame deletion of ccpN gene.cThe first double-crossover recombination efficiency was calculated as the number of CmR colonies/µg of dsDNA PCR product.dCounter-selection efficiency was calculated as Nr/Nt. Nr, number of 5FUR CmS colonies in the culture treated by different concentration of xylose; Nt, number of total colonies in culture treated by different concentration of xylose.