Transcriptome Profiling in tissues and blood from NHP-HCT Recipients

Tissue-specific T cell immune responses play a critical role in maintaining organ health, but can also drive immune pathology during both auto- and alloimmunity. The mechanisms controlling intra-tissue T cell programming remain unclear. Here, we leverage a non-human primate model of acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (HCT) to probe the biological underpinnings of tissue-specific alloimmune disease, using a comprehensive systems immunology approach.Transcriptional profiling revealed substantial biological differences between T cells, infiltrating the lung and liver during aGVHD. Series of HCT performed on n=13 animals with n=37 arrays from the blood and tissue in the post transplant period including arrays from n=42 untransplanted healthy controls