Next Generation Sequencing for Chromatin accessbility by ATAC-seq for MDA-MB-231, MCF7 or HCT116 cell line

CRISPRi on PVT1 did not induce drastic changes on chromatin accessbility in MDA-MB-231 cell line. CCAT1 enhancer is accessble specifically in HCT116, not in MCF7 or MDA-MB-231. MDA-MB-231 cell line was treated non-targeting control sgRNA or PVT1 targeting sgRNA. All cell lines express dCas9-BFP-KRAB integrated via lentivirus.