RNA-seq muscle (AR)

Identify the genes modulated by Androgen Receptor. Overall design: We selectively knocked-out AR in myofibers by crossing AR floxed mice to HSA-Cre mice. RNA from gastrocnemius muscle of 9 week-old control (AR floxed mice) and knock-out mice was extracted with trizol according to the manufacturer protocol. RNA samples were sequenced by the standard Illumina protocol to create raw sequence files (.fastq files). We annotated these reads to the mm10 build of the mouse genome using HOMER. The aligned reads were counted with the homer software (analyze RNA) and DEG's were identified using EdgeR and DESeq.