IL-8 Reporter Assay in LPS-stimulated and unstimulated cystic fibrosis airway cells treated with WLBU-2 or LL-37

CF IB3-1 cells (unstimulated and LPS-stimulated) were transfected with a 5' firefly luciferase gene flanking a 200bp wild type- or dNFκB IL-8 promoter and treated with 25µM WLBU-2 or LL-37 followed by measurement of IL-8 promoter activity at time points ranging from 30min-4h by Dual-Luciferase Reporter assay. PBS was used as a control in LPS-stimulated and unstimulated cells. Data represent means/standard deviations of the count readings and calculations of fold-change relative to the PBS-treated controls.

CF IB3-1 细胞（未经刺激和经脂多糖刺激）被转染了位于200bp野生型或dNFκB IL-8启动子两侧的5'萤火虫荧光素酶基因，随后用25µM WLBU-2或LL-37进行处理，并采用Dual-Luciferase Reporter试剂在30分钟至4小时的时间点测量IL-8启动子活性。在脂多糖刺激和未经刺激的细胞中，使用磷酸盐缓冲盐溶液（PBS）作为对照。数据表示计数读数的平均值及标准差，以及相对于PBS处理对照组的倍数变化计算。