Raw data of Cedar Creek Experiment

<strong>1.-Experiment </strong> The experimental design consisted of 21 populations inoculated with 1 mL water samples taken near the Cedar Bog Lake dock on July 7th. Each population consisted of a final volume of 39 mL in transparent Greiner Bio-One vented-cap culture flasks. We used a three-by-two factorial design with three nitrogen concentrations (standard BG11 medium (17.6 mM NaNO3), a reduced nitrogen BG11 medium (9 mM NaNO3), and a BG11-0 medium (without nitrogen)) and (10 mM NaCl) and without NaCl addition. To account for the effect of nutrient addition, we added a control treatment with deionized water. Three replicates population of each experimental condition were stabilized. The experiment was run for 3 weeks. Table 1: Experimental design. <strong>dH20</strong> <strong>BG11</strong> <strong>BG11 reduce Nitrogen</strong> <strong>B11 without Nitrogen </strong> <strong>BG11 +NaCl</strong> <strong>BG11 reduce Nitrogen</strong> <strong>+NaCl</strong> <strong>B11 without Nitrogen+NaC</strong>l R1 R16 R10 R4 R19 R13 R7 R2 R17 R11 R5 R20 R14 R8 R3 R18 R12 R6 R21 R15 R9 <strong>1.1 FlowCam( Fluid Imaging Technologies) Data collection</strong> 0.15 mL aliquots of each population at week three were run through a Flowcam cytometer (Fluid Imaging Technologies) at a speed of 0.14 mL/min and collect images of individual particles and estimate biovolume using total area. The 10x magnification setting was used with a calibration factor of 0.5525. For each sample, the first 1,000 images taken by FlowCam were selected for species diversity analysis. Each folder is a separate population at T3 (21 days). It contains the original run (pictures and files) and within it can be found the classification by “groups” (pictures and files). <strong>1.2 Flotation Device Technical Description</strong> Flotation Device is a suspension system to safely maintain planktonic microbial populations to carry out studies at the water temperature and light conditions of a water body. <strong>1.3 Bradford Protein Assay Data </strong> Populations were checked weekly by sampling aliquots of all the populations and running Bradford Protein Assays as a proxy for population growth.