RNA sequencing to detect the differentially expressed genes in AngII infused mice model after QDG treatment.

The mice (n=15) were randomly divided into 3 groups: Control, Ang ?, and Ang ?+QDG groups (n = 5 for each group). Mice in Control and Ang ? groups were infused with saline or 500 ng/kg/min of Ang ? respectively, and orally administrated with saline; while mice in Ang ? + QDG group were infused with Ang ? (500 ng/kg/min) and orally administrated with 1.145 g/kg /day of QDG for 2 weeks. Then the cardiac tissues were used to identify differentially expressed genes among different groups. Overall design: RNA sequencing was performed with heart tissue of control, AngII and QDG treated (n = 5 for each group ) mice to identify the differently expressed genes and related pathway.