Late cornified envelope 1C (LCE1C) gene is a transcriptional target of TAp63.

As a member of the p53/p63/p73 family, p63 is a transcriptional regulator that induces apoptosis, cell cycle arrest, or senescence. TAp63 protein, a p53-like master transcriptional regulator, plays an essential role in epithelial development because p63-null mice are born alive but die shortly after birth due to developmental defects. However, its molecular mechanism remains elusive. We identified these phosphorylation sites during a search for the targets of Cyclin G-associated kinase (GAK) in vitro. LCE1C was drastically upregulated by doxycycline-dependent expression of Myc-TAp63 wild-type (WT) proteins, but not non-phosphorylatable Myc-TAp63-T46A/T281A (AA) proteins. Gene expression analysis of Tet-ON inducible Myc-TAp63-WT or Myc-TAp63-T46A/T281A (AA) U2OS cells with (+) or without (-) doxycycline (Dox) was performed using Agilent Human Gene Expression 4x44K v2 Microarray as one color experiments.