Data from experimental microscope showing GCaMP6f fluorescence in neuronal mouse organoids

This dataset supports the study of a low-cost, modular fluorescence microscope designed for high-resolution, wide field-of-view, high frame-rate, and multi-channel live cell imaging. The data includes TIFF images, video files, and CSV files of analyzed results, demonstrating the system's ability to capture live neuronal activity in forebrain organoids using GCaMP6. The study employs both GCaMP6 and mScarlet to showcase multi-channel fluorescence. This platform offers a novel, cost-effective alternative to traditional systems, providing enhanced flexibility and performance for live cell fluorescence microscopy. The initial publication of this data accompanies an anticipated 2025 research article authored by Nicholas "Nico" Hawthorne et al.

The image stack represents raw data collected from the camera. A shorter section with vibrant activity was chosen for processing, performed in Image-J, using automatic contrast adjustment and application of a green look up table. Regions of interests (ROIs) were identified using the methodology described in the paper. ROIs.csv was generated indicating the average pixel intensities of the regions believed to have firing neurons. One movie file generated from the raw data and one movie file generated from the processed data are included in order to easily visualize the datasets.