Richard Allen (2010) CIL:9713, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Bundle of microtubules supporting the oral apparatus of Coleps. This micrograph shows the detail of microtubular cross-bridging within one of these rods. Microfibrillar bridges are located at 22nm intervals within the hexagonally packed microtubules. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/29/69 by R. Allen with Philips 300 operating at 60kV. Neg. 63,750X. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:2882) is available for quantitative analysis.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

一束支持 Coleps 口器的微管。此显微照片展示了这些棒状物中微管交叉桥接的细节。在六角紧密排列的微管中，微纤维桥以22纳米的间隔分布。采用标准戊二醛固定，随后进行四氧化锇处理，以酒精脱水，并嵌入环氧树脂中。制备的微切片厚度约为75纳米。1970年5月29日，由R. Allen使用Philips 300型电镜，在60kV的电压下进行透射电镜成像。负片放大倍数为63,750倍。负片打印件经过扫描并在Photoshop中处理。此图像适用于定性分析。高分辨率图像（CIL:2882）可用于定量分析。