Source Data Figure 2 .xlsx

b, The integrated optical density (IOD) value for proteoglycan
content determined by safranin O-fast green staining (n = 8 for each group; one-way ANOVA). c, Proteoglycan staining area (%) determined by safranin O-fast
green staining (n = 8 for each group; 2 w, one-way ANOVA; 3 w, nonparametric
test). e, The mRNA levels of
anabolic and inflammatory biomarkers in OA cartilage explants analyzed by reverse
transcription polymerase chain reaction (RT-PCR) after BNTA treatment (0.1 μM) for
3 w (n = 5 for each group). proteoglycan 4 (PRG4),
SRY-box 9 (SOX9), and interleukin 6 (IL6), unpaired two-tailed Student’s t-test; collagen type II alpha 1 chain (COL2A1), aggrecan
(ACAN), heparan sulfate
proteoglycan 2 (HSPG2), and C-C motif chemokine ligand 2 (CCL2), nonparametric test. f,
glycosaminoglycan (GAG) and DNA content per wet weight was assessed by 1,
9-dimethylmethylene blue (DMMB) and Hoechst 33258 assays after vehicle or BNTA treatment
for 2 w, separately (n = 6 for each group; nonparametric test). Data are shown
as the mean ± standard deviation (s. d.). *P
< 0.05, **P < 0.01, ***P < 0.001.