Transcriptome analysis of control and RBM6 KO cells before and after DNA damage

Control and RBM6-KO MCF10A-Hras cells were subjected to RNA-sequencing before and after 12hrs exposure to 5Gy ionizing radiation (IR). Transcriptome analysis revealed a subset of genes that are differentially regulated in RBM6-KO cells compared to control cells. In addition, transcriptome of RBM6-KO cells after IR showed upregulation of DNA damage genes, suggesting impaired DNA repair compared to control cells. RNA was purified from two biological replicates of control and RBM6 KO MCF10A-Hras cell line before and after 12hrs recovery from 5Gy IR. (4 conditions with two replicates each)