DNA microarray analysis using duodenum from wild-type or IAP knockout (Akp3-/-) mice administered with IIAEK (Ile-Ile-Ala-Glu-Lys)

To understand the molecular mechanism by which IIAEK ameliorates hepatic and intestinal cholesterol metabolism, we performed DNA microarray analysis using liver, duodenal, and jejunal samples from the control and IIAEK groups of WT or IAP KO mice. In total, 1406 transcripts were identified in the duodenum of WT mice ( ≥1.2 fold-change, p < 0.05). In contrast, we identified 777 transcripts in the duodenum of IAP-KO mice ( ≥1.2 fold-change, p < 0.05). Seven week-old wild type (WT) and IAP knockout (IAP-KO) (Akp3-/-) mice were administered orally the pentapeptide IIAEK (Ile-Ile-Ala-Glu-Lys) (600 mg/kg B.W./d) once a day for 14 d. After fasting for 8 h, the duodenum was collected from the mice for DNA microarray analyses (n=3/group).