Free-floating Long-term Vascularized Mesenchymal Organoids [scRNA-seq]

The vasculature is essential for tissue function and pathology. Spheroid co-cultures of endothelial and mesenchymal stem/stromal cells (MSCs) form consistent structures, but the vascular components are short-lived. iPSC-derived vascular organoids can establish complex vasculature but often have cell heterogeneous variable maturation and low reproducibility. We presents consistently-formed, free-floating, long-term Vascularized Mesenchymal Organoids (VMOs), formed by co-culturing human umbilical vein endothelial cells (HUVECs) and MSCs in a pre-gelled minimal Matrigel scaffold. We studied, MSC heterogeneity, vascular biology and system dynamics. Overall design: Samples are cells extracted and pooled from digested organoids cultured for 3 days and 14 days respectively