An in vitro study of the development of resistance to FGFR inhibition in two urothelial carcinoma cell lines

Expression array data was used to compare parental FGFR3-TACC3 fusion-driven urothelial cell lines with their FGFR inhibitor-resistant derivatives. In this dataset, we include RT112 and RT4 parental cells, RT112 cells acutely treated with PD173074 (24 h), RT112 and RT4 resistant derivatives cultured with drug and their resistant derivatives cultured for four to six passages out of drug. 11 cell lines were analysed on the Affymetrix HTA 2.0 platform: RT112 and RT4 parental cells (RT112 parental no PD; RT4 parental no PD), RT112 cells acutely treated with PD173074 (24 h) (RT112 parental PD), RT112 and RT4 resistant derivatives cultured with drug (RT112 R1 PD; RT112 R2 PD; RT112 R3 PD; RT4 R1 PD) and their resistant derivatives cultured for four to six passages out of drug (RT112 R1 no PD; RT112 R2 no PD; RT112 R3 no PD; RT4 R1 no PD). Each cell line was analysed in triplicate (33 samples in total).