LncRNA Malat1 suppresses pyroptosis and T cell-mediated killing of incipient metastatic cells [RNA-seq_4T1]

Malat1 was KO using paired gRNA CRISPR-cas9 system from 4T1 cells. Overall design: Total RNA was isolated using the Maxwell instrument from 4T1 WT or Malat1 KO cells and sent to BGI for RNA-seq. The sample name was annotated below. 1. 4T1_Ctrl-1: 4T1-Ctrl-1 2. 4T1_Ctrl-2: 4T1-Ctrl-2 3. 4T1_Ctrl-3: 4T1-Ctrl-3 4. 4T1_Malat1_KO1-1: 4T1-KO#3-1 5. 4T1_Malat1_KO1-2: 4T1-KO#3-2 6. 4T1_Malat1_KO1-6: 4T1-KO#3-3 7. 4T1_Malat1_KO2-1: 4T1-KO#43-1 8. 4T1_Malat1_KO2-2: 4T1-KO#43-2 9. 4T1_Malat1_KO2-1: 4T1-KO#43-3