Targeted cross-linker delivery for the in situ mapping of protein conformations and interactions in mitochondria

The size, PDI, zeta potential and NTA of the NPs were determined using a Zetasizer Nano-ZS instrument (Malvern instruments, UK), and each measurement was run at three times using automated, optimal, measurement times and laser attenuation settings. The loading efficiency was measured using a HITACHI Chromaster high-performance liquid chromatography (HPLC, Japan) instrument, and each measurement was run at three times. The labeling efficiency of the NPs with fluorescent reagents was determined using an SH800S cell sorter (Sony Biotechnology). The fluorescence image were taken by laser scanning confocal microscopy (Nikon) and Andor live cell confocal imaging platform (Revolution WD). <br>