monoallelic expression and methylation (Bombus terrestis)

we examined the link between monoallelic methylation and monoallelic expression in thebumblebee, Bombus terrestris, by examining two whole methylome libraries and an RNA-seq library from the same bee. MeDIP-seq is an immunoprecipitation technique that creates libraries enriched for methylated cytosines (Harris et al., 2010). Methyl-sensitive restriction enzymes can create libraries that are enriched for non-methylated cytosines (MRE-seq) (Harris et al., 2010). Genes found in both libraries are monoallelically methylated, with the hypermethylated allele being in the MeDIP-seq data and the hypomethylated allele in the MRE-seq data (Harris et al., 2010). Monoallelic expression was identified in these loci from the RNA-seq library. If only one allele was expressed then we knew that these loci were both monoallelically methylated and monoallelically expressed in this bee.