Mytilus galloprovincialis 4 tissues x 5 salinities x 2 times

Summary statistics and pairwise comparisons for biochemical biomarkers measured in different tissues of Mytilus galloprovincialis. Mean (±SD) values and p-values for statistical comparisons between experimental conditions. For each biomarker, differences were tested using either the Student’s t-test (for normally distributed data) or the Wilcoxon rank-sum (Mann–Whitney) test (for non-parametric data). “Time comparison” corresponds to 14 vs 28 days of exposure at the same salinity. “Salinity vs 30” corresponds to each salinity level (20, 25, 35, or 40) compared with the reference salinity (30) at the same exposure time. Significant differences (p < 0.05) are highlighted in the main figures. Abbreviations Tissues DG – Digestive gland G – Gills MU – Muscle MA – Mantle Functional groups Metabolism – Enzymes and indicators related to energy metabolism and reserves. Antioxidant system and biotransformation – Enzymes involved in antioxidant defense and detoxification. Cellular damage – Biomarkers of oxidative or membrane damage. Neurotoxicity – Enzyme related to neuronal function and inhibition by contaminants. Biochemical biomarkers SDH – Succinate dehydrogenase activity (mitochondrial metabolism) ETS – Electron transport system activity (overall metabolic capacity) PROT – Soluble protein content GLY – Glycogen content SOD – Superoxide dismutase activity GPx – Glutathione peroxidase activity GR – Glutathione reductase activity GSTs – Glutathione S-transferases activity TAC – Total antioxidant capacity CbEs – Carboxylesterases activity LPO – Lipid peroxidation (oxidative damage indicator) AChE – Acetylcholinesterase activity (neuromuscular function marker) Column descriptions Functional Group: Biochemical category of the biomarker (Metabolism, Antioxidant, CellDamage, or Neurotoxicity). Tissue: Organ analyzed (DG, G, MU, MA). Biomarker: Specific biochemical parameter measured. Salinity / Salinity (vs 30): Salinity level (20, 25, 30, 35, 40) compared with reference salinity (30). Time: Exposure time (14 or 28 days). mean_14 / mean_28: Mean value at 14 or 28 days. sd_14 / sd_28: Standard deviation corresponding to each mean. mean_30 / mean_comp: Mean values for salinity 30 and for the compared salinity, respectively. sd_30 / sd_comp: Standard deviations corresponding to each mean. Time p / Salinity p: p-value for the statistical comparison. Test Used: Statistical test applied (t-test or Wilcoxon-Mann–Whitney).