Next Generation Sequencing Analysis of Wild Type (WT) and SRSF1 knockdown (KD) U87MG and U251 cells Transcriptomes

We generated the SRSF1 knockdown U87MG and U251 cells by infecting with shRNA virus. Then, we extracted RNAs and performed next generation sequencing. By comparing sequencing data from WT and KD samples in each cell type, we profiled the alternative splicing events and gene expression regulated by SRSF1 in GBM. WT and KD groups of U87MG and U251 cells mRNA profiling was performed by deep sequencing, using Illumina HiSeq2000.