Ip, Wallace (2010) CIL:2880, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in 0.2% saponin. The fibers were quick-frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:2879.

本研究中，兔子的腰大肌肌纤维在0.2%的皂苷溶液中保持恒定长度并经过皂苷诱导的僵直处理。肌纤维通过接触液氦冷却的铜块在Heuser型冷冻压机上迅速冷冻，并在Balzers 400冷冻断裂机上进行冷冻蚀刻。随后，样品在24摄氏度下采用Pt-C进行旋转复制，并在运行于100 kev的JEOL 100CX2透射电子显微镜下进行观察。图像以75,000倍放大率记录在Kodak 4489胶片上，并在+5度和-5度处拍摄立体对。胶片使用Nikon Coolscan 9000ED扫描仪进行扫描，像素间距为20微米。与CIL:2879的立体对图像一同进行扫描。