MUSCLE (MUltiplexed Single-molecule Characterization at the Library scalE) protocol data and codes

A test dataset for MUSCLE (MUltiplexed Single-molecule Characterization at the Library scalE) data analysis. See "\Python codes for MUSCLE data analysis\README.txt" for the instructions on running the data analysis codes. Use the files in the "Test MUSCLE dataset" folder as input for the codes. "Test MUSCLE dataset\Output_tile1\" contains the code output for the test dataset. The example dataset corresponds to one MiSeq tile in an experiment analyzing dCas9-induced R-loop formation for a library of 256 different target sequences.The latest version of the Python codes for matching single-molecule FRET traces with sequenced clusters is available at https://github.com/deindllab/MUSCLE/.