A Simplified Preparation Method for Single-Nucleus RNA-Sequencing Using Long-Term Frozen Brain Tumor Tissues

Due to an increasing need for advanced techniques to understand the heterogeneity of central nervous system tumors, we optimized a method to isolate intact nuclei from long-term frozen pediatric brain tumor tissues. The results demonstrated that the protocol is fast, simple and low-coust thus optinal for potential application for such cases. A set of long-term frozen tissues from pediatric brain tumors were processed with a specific single nuclei protocol in order to evaluate the suitability of the protocol for studing tumor heterogeneity *************************************************************** Submitter states that missing raw files are due to patient privacy concerns. ***************************************************************