Specific deshydrogenase activity of the recombinant ceQORH protein.
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A. The deshydrogenase activity of the purified recombinant HisceQORH proteins was measured as described in materials and methods. The impact of the lack of every reaction components was tested. The specific activity determined for the HisceQORH protein (100%) was 19.3 nmoles of formazan mg/ceQORH protein/min. B. Dehydrogenase activity of the ceQORHnh protein and of the ceQORHatt protein (containing the N-terminal “att” extension) produced in L. lactis and the ceQORH protein produced in E. coli.
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2010-01-20
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