Single-cell multiomics sequencing (RNA, ADT and VDJ) of CD4+ T cells from peripheral blood of CMV+ and CMV- healthy humans.

The goal of this study is to understand the transcriptional features of CD4+ memory T cells induced by CMV seropositivity and explore their TCR repertoire clonality. Overall design: We selected CMV-associated CD4+ T cells based on ADT expression matrix. Differential expression analysis based on gene expression matrix was performed to extract transcriptiomes upregulated within these cells. Clonotypes of CD4+ T cells were assigned based on their unique combination of paired V(D)J gene usage and paired productive CDR3 sequences of alpha and beta chain. Simpson's Reciprocal Index was calculated to compare the clonal diversity of CMV-associated CD4+ T cells with other T cell subsets.