Quantitative Analysis of Worms after P. aeruginosa Infection

To clarify the mechanisms of innate immune responses mediated by epithelial barrier disruption. Reveal the host's broad transcriptional response to P. aeruginosa infection during disruption of the intestinal barrier. We used RNA-Seq analysis to study the transcriptomic profiles of wt worms were exposed to either P. aeruginosa PA14 or E. coli OP50 for 24 hours, and the transcriptomic profiles of egl-44(MT2247) mutants fed E. coli OP50 for 24 hours. Differential expression analysis was performed by comparing wt worms exposed to PA14 versus wt worms exposed to E. coli OP50, and egl-44(MT2247) mutants fed E. coli OP50 versus WT worms fed E. coli OP50. Overall design: Young adult wt worms were exposed to either P. aeruginosa PA14 or E. coli OP50 for 24 hours, and young adult egl-44(MT2247) mutants fed E. coli OP50 for 24 hours. Bacteria were removed and worms were natural precipitation by M9 buffer , flash frozen on dry ice. The transcriptional profiles of those samples were generated by deep sequence, in triplicate, using Illumina Novaseq™.